Identification bacteria with maldi-tof 😀 The principle of MALDI After a very brief laser pulse, the irradiated spot is rapidly heated and becomes vibrationally excited. The matrix molecules energetically ablated from the surface of the sample, absorb the laser energy and carry the analyte molecules into the gas phase as well. The MALDI-TOF technique can assess Gram-positive, Gram-negative, molds, mycobacteria, and anaerobic microbes, providing detailed information necessary to accurately identi